the potential of human-derived periodontal ligament stem cells to osteogenic differentiation: an in vitro investigation

نویسندگان

behzad houshmand department of periodontics, school of dentistry, shahid beheshti university of medical sciences, tehran, iran

omolbanin amjadi molecular and cell biology research center, department of immunology, school of medicine, mazandaran university of medical sciences, mazandaran, iran

alireza rafiei molecular and cell biology research center, department of immunology, school of medicine, mazandaran university of medical sciences, mazandaran, iran

mohammadali rouzegar department of periodontology, school of dentistery, ilam university of medical sciences, ilam, iran

چکیده

background: periodontal ligament stem cells (pdlscs) are considered as a type of mesenchymal stem cell that is beneficial target for numerous clinical applications in periodontal tissue regeneration therapy. materials and methods: this study examined the effects of dexamethasone (dex) on human pdlscs in vitro. pdlscs obtained from the roots of patient’s teeth were cultured with dex (0.01 μm), and their proliferation was measured. the osteogenic differentiation was assessed by alkaline phosphatase (alp) activity and alizarin red-s staining for calcium deposition. results: after the administration of 0.01 μm dex, the activity of alp increased significantly. furthermore, mineralized nodule formation showing the intracellular calcium deposition was significantly higher in the dex-treated cells than that of the control cells. conclusion: collectively, dex has positive effects on osteogenic differentiation of human pdlscs in vitro. it is suggested that pdlscs may serve as a potential material for periodontal tissue regeneration.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The potential of human-derived periodontal ligament stem cells to osteogenic differentiation: An In vitro investigation

Background: Periodontal ligament stem cells (PDLSCs) are considered as a type of mesenchymal stem cell that is beneficial target for numerous clinical applications in periodontal tissue regeneration therapy. Materials and Methods: This study examined the effects of dexamethasone (Dex) on human PDLSCs in vitro. PDLSCs obtained from the roots of patient’s teeth were cultured with Dex (0....

متن کامل

Curcumin promotes osteogenic differentiation of periodontal ligament stem cells through the PI3K/AKT/Nrf2 signaling pathway

Objective(s): The aim of this study was to investigate the effect of curcumin on the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and its underlying potential mechanism.Materials and Methods: The tissue explant adherence method was used to isolate hPDLSCs. Flowcytometry, Alizarin Red staining and Oil Red ...

متن کامل

assessment of surface markers derived from human periodontal ligament stem cells: an in vitro study

objectives: periodontal tissue regeneration for treatment of periodontal disease has not yet been mastered in tissue engineering. stem cells, scaffold, and growth factors are the three main basic components of tissue engineering. periodontal ligament (pdl) contains stem cells; however, the number, potency and features of these cells have not yet been understood. this study aimed to isolate and ...

متن کامل

Reprogramming by cytosolic extract of human embryonic stem cells improves dopaminergic differentiation potential of human adipose tissue-derived stem cells

The extract of pluripotent stem cells induces dedifferentiation of somatic cells with restricted plasticity. In this study, we used the extract of human embryonic stem cells (hESC) to dedifferentiate adipose tissue-derived stem cells (ADSCs) and examined the impact of this reprogramming event on dopaminergic differentiation of the cells. For this purpose, cytoplasmic extract of ESCs was prepare...

متن کامل

The Potential of Menstrual Blood-Derived Stem Cells in Differentiation to Epidermal Lineage: A Preliminary Report

BACKGROUND Menstrual blood-derived stem cells (MenSCs) are a novel source of stem cells that can be easily isolated non-invasively from female volunteered donor without ethical consideration. These mesenchymal-like stem cells have high rate of proliferation and possess multi lineage differentiation potency. This study was undertaken to isolate the MenSCs and assess their potential in differenti...

متن کامل

Gene expression pattern during osteogenic differentiation of human periodontal ligament cells in vitro

PURPOSE Periodontal ligament (PDL) cell differentiation into osteoblasts is important in bone formation. Bone formation is a complex biological process and involves several tightly regulated gene expression patterns of bone-related proteins. The expression patterns of bone related proteins are regulated in a temporal manner both in vivo and in vitro. The aim of this study was to observe the gen...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
research in molecular medicine

جلد ۲، شماره ۴، صفحات ۱۸-۲۳

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023